Biology: Biochemistry and Molecular Biology: Methods and Techniques: PCR

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	Home: Biology: Biochemistry and Molecular Biology: Methods and Techniques: PCR CATEGORIES:   Amplified Fragment Length Polymorphism (7)   In Situ PCR (9)   Real Time PCR (13)   RNA to DNA Amplification (9)   Software (9) LINKS:   Pages: 1 2 3 [>>] Roe Laboratory Protocols http://www.genome.ou.edu/proto.html Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK. Design Oligonunucleotide Primers http://sciborg.uwaterloo.ca/~mpalmer/silent_mutations.html Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada. FISH Guide and Troubleshooting http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT. Protocol for T-RFLP by Capillary Electrophoresis http://rdp.cme.msu.edu/html/t-rflp_jul02.html Detailed protocol for terminal restriction fragment length polymorphism (T-RFLP), which allows the fingerprinting of a community by analyzing the polymorphism of a certain gene. Article by V Grüntzig, B Stres, HL Ayala del Río and JM Tiedje, Center for Microbial Ecology, Michigan State University, USA The web guide of PCR http://www.pcrlinks.com/ List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina. Degenerate PCR http://www.dartmouth.edu/artsci/bio/ambros/protocols/other/koelle/degenerate_PCR.html The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College. Long PCR Protocol http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA DNALC: PCR Animation http://www.dnalc.org/shockwave/pcranwhole.html An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory. HiFi DNA http://www.hifidna.com/ HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails. Kary B. Mullis - Autobiography http://www.nobel.se/chemistry/laureates/1993/mullis-autobio.html The originator of PCR, from the Nobel e-museum web site. PCR Project http://sunsite.berkeley.edu/biotech/pcr/ Presentations from the University of California at Berkley on PCR, both current research reports and reviews. PCR Protocols http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University PCR Technology http://www.accessexcellence.org/LC/SS/PS/PCR/PCR_technology.html An introduction by Connie Veilleux from the US National Health Museum website. The Polymerase Chain Reaction http://www.faseb.org/opar/bloodsupply/pcr.html A popular description of the way it works, its importance in different connections, legal aspects. Authored by TM Powledge at the Federation of the American Societies of Experimental Biology. Polymerase Chain Reaction (PCR) http://www.accessexcellence.org/AB/GG/polymerase.html A graphic description of the principle of PCR from the US National Health Museum web site. Principle of PCR http://allserv.rug.ac.be/~avierstr/principles/pcr.html Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium. Rep-PCR Genomic Fingerprinting http://www.msu.edu/user/debruijn/ Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions. Tavi's PCR protocols http://info.med.yale.edu/genetics/ward/tavi/PCR.html A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful. Technical manual of PCR http://web.uct.ac.za/microbiology/pcr.htm Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001. What the Heck is PCR? http://people.ku.edu/~jbrown/pcr.html Popular description of the PCR technique by John C Brown, University of Kansas 1995. Polymerase Chain Reaction http://www.accessexcellence.com/RC/CT/polymerase_chain_reaction.html Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum. Kary Mullis http://www.invent.org/hall_of_fame/109.html Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site. Wayward PCR primers http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jan95.html Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time. Fidelity of DNA Polymerases for PCR http://www.lecb.ncifcrf.gov/~pnh/papers/TIBS/aug95.html Article by PN Hengen from TIBS 1995 PCR protocols http://genome.wustl.edu/tools/protocols/index.php?fin4=1 Brief protocols for PCR in use at the Genome Sequencing Center of the Washington University in St Louis Medical School.   Pages: 1 2 3 [>>]

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